Bioremediation of oily waste / sludge generated by petroleum industry, stry, extraction of oil by Soxhlet Method and its Analysis by GC Method

摘要

A field scale study was conducted in a land contaminated with an oily sludge by use of a carrier based hydrocarbon- degrading consortium for bioremediation.The land belonged to oil refinery. Prior to this study a feasibility study was conducted to access the capacity of bacterial consortium to degrade oily sludge. At GGS-I Kalol, GGS-I Limbodara, GGS-II Limbodara and GGS Gamij pits were undertaken for bioremediation. In this study consortium was prepared in large scale in bioreactor and was used in field area. Samples of the soil at zero day (without application of microbial consortia) and after application of microbial consortia at different time interval period i.e. one month two and three months till complete degradation of oily sludge/ soil is completed was collected. Soil samples of the site were subjected for oil extraction using soxhlet method. Weight of the oil was calculated by simple gravimetric method. Column fractionation was done for the extracted oil before injecting in gas chromatogram. Characterization of oil was done by (Gas Chromatograph (Hewlett Packard 5890 SeriesII using, GC Column (DB2887) for Alkane and Column (DB23) for Aromatic samples, ~1H, spectra in CDCl_3 were obtained in Bruker DRX-400 instrument (Carlsruhe, Germany solid-state ~(13)C NMR spectra were obtained with Brucker DRX-400 MHz andsmm mass probes. All ~(13)C spectra were run on a samples spinning at 28735±2Hz. The 'H-NMRwas measured at a spinning speed of 5592±iHz).