SURFACE PLASMON RESONANCE (SPR) BIOSENSOR FOR GENETICALLY MODIFIED ORGANISMS (GMOs) DETECTION

摘要

The development of a Surface Plasmon Resonance (SPR) affinity biosensor based on DNA hybridisation is described. This biosensor has been applied to Genetically Modified Organisms (GMOs) detection. Single stranded DNA (ssDNA) probes were immobilised on the sensor chip of an SPR device and the hybridisation between the immobilised probe and the complementary sequence (target) was monitored. The probe sequences were internal to the 35S promoter and NOS terminator sequences which are inserted in the genome of GMO regulating the transgene expression. The system has been optimised using synthetic oligonucleotides, then applied to real samples analysis. Samples, containing the transgenic target sequences, were amplified by Polymerase Chain Reaction (PCR) and then detected with the SPR biosensor.