Identification of Peroxisome Proliferation BioMarkers by SELDI/ProteinChip Arrays

摘要

1. SELDI/ProteinChip Array system was evaluated for bio-marker identification using wild-type, AOX~(-/-), and Wy14,643 induced mouse liver samples. 2. Screened by four different chip surfaces, the liver protein profile of AOX-/- mice showed similarity with Wy14,643 induced mice, however, significant difference from wild-type mice. 3. A total of forty peaks showed more than 2-fold difference between wild type and treated mouse liver samples. 4. The 18.7-kDa protein was enriched by Q-Saphadex spin column and further purified from SDS-PAGE gel for tryptic digestion. 5. As a proof of principle, tryptic mapping by SELDI identified the 18.7 kDa protein as major urinary protein, which was further confirmed by LC-MS/MS.