DETERMINATION OF L-GLUTAMATE ACID WITH AN IMMOBILIZED ENZYME COLUMN REACTOR

摘要

L-glutamate is present in many physiological fluids and pays an important role in ammo acid biosynthesis and metabolism. Usually, the determination of L-glutamic acid is based on chromatographic techniques, but the method is unsuitable for the rapid analysis of large numbers of samples. The use of immobilized enzymes in analytical chemistry in recent year has been stimulated by their advantage. The key problem of their applications is the immobilization of enzyme on solid supports. The object of this study is to develop a sensitive method for the determination of L-glutamic acid in ferment solution using a new biosensor which consists of an enzyme column reactor of L-glutamic decarboxylase (GDC) immobilized on a novel ion exchange resin (carboxymethyl-copolymer of allyl dextrin and N,N'-methylene-bisarylamide, CM-CADB) and the CO_2 electrode.