In this project, we have optimized the parameters for PAH degradation by the white rot fungi Bjerkandera sp. strain BOS55 and Pleurotus ostreatus. Bjerkandera sp. strain BOS55 was previously shown to be an outstanding PAH degrading isolate, which could degrade PAHs at extremely high rates in liquid cultures. In artificially contaminated soils, benzo[a] pyrene was also rapidly degraded by Bjerkandera sp. strain BOS55, with only low residual concentrations. In a size-fractionated soil, the degradation rate of benzo[a] pyrene degradation could be enhanced by the addition of surfactants (1-7.5 g L~(-1)). The performance of both white rot fungi was then tested in bench-scale experiments with aged PAH-contaminated soils. At low, economical amounts of 15
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