Gene transfer in zebrafish enhanced by nuclear localization signals

摘要

Transgenic fish are routinely produced by injection of plasmid DNA into eggs (reviewed by Flechter and Davies, 1991). In zebrafish, the pronuclei of fertilized eggs are not visible, thus the DNA is injected into the cytoplasm or the yolk. Cytoplasmically injected DNA can integrate into the genome and be transmitted through the germline (Stuart et al., 1988; 1990). Frequencies of transgene integration and germline transmission may be as high as 25percent (Stuart et al., 1988; Culp et al., 1991) but are often in the order of a few percents.