The Isolation and Proliferation Characteristics of Rabbit Adipos Derived Stromal Cells at Early Stage in Vitro

摘要

AIM: The objective of this study was to identify and analyze the isolation and proliferation characteristics of early stage rabbit adipos derived stromal cells at early stage in vitro, including the morphology and Clone-forming capacity.METHODS: Adipose tissue was harvested from the nape fat pad of the female New Zealand rabbits. Primary cultures were established using type I collagenase and cell cultures were maintained with DMEM+10% FBS. The morphological characterization of adipos derived stromal cells at early stage was described. Expressions of CD29 and Proliferating Cell Nuclear Antigen were analyzed by flow cytometry. The Cloning capacity was determined using a ring cloning procedure. Data was statistically analyzed by software SPSS 16.0.RESULTS: ADSCs were successfully obtained and maintained which appeared fibroblast-like and proliferated rapidly in vitro. Flow cytometry showed that ADSCs expressed CD29 and PCNA. The colone-forming rate of passage 2 was (8.0±0.6)%, while they were (6.7±0.4)% and (4.6±0.5)% at passage 3 and passage 4, respectively. Statistical analysis indicated that the significant difference among the ADSCs at early stage.CONCLUSION: Rabbit ADSCs at early stage can selfrenew and proliferate rapidly in vitro. The colony formation capacity was gradually decreased obviously with passage number increased.