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SPORE TRAP DETECTION OF SUGARCANE SMUT: 12 MONTHS EXPERIENCE AND OUTCOMES

机译:孢子捕获甘蔗渣的捕获检测:12个月的经验和结果

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SUGARCANE SMUT was first detected in Queensland in the Childers-Bundaberg region in June 2006. Crop inspections revealed over 70 farms were smut-infested by November 2006. With the disease found in the Mackay and Herbert districts by the end of 2006,the likelihood of smut infestations in other apparently un-infested areas became much more likely. Burkard spore traps were purchased to assist in detecting smut in these districts. Initial trials involved placing the traps close to infested crops and assaying spore capture using a light microscope but light microscopy proved time consuming and costly from a time perspective. A molecular assay was developed to speed the process and although not providing a quantitative assay result, it still provided aqualitative assessment of the presence of smut. Spore trapping was undertaken in a number of crops in many of the un-infested cropping areas in Queensland and New South Wales. Smut-positive assay results for U. scitaminea spores were recorded in the Mossman, Burdekin, Proserpine and Maryborough areas. High intensity trapping at some of these sites showed that U. scitaminea spores were common and that the disease is very likely to be infesting crops in at least some of these locations. Smut whips were seen for the first time in the Maryborough area on 22 November 2007.
机译:甘蔗Smut于2006年6月在童年南巴格地区的昆士兰州检测到。作物检验显示70多个农场,于2006年11月举行了耻辱。在2006年底,麦田和赫伯特地区发现的疾病是可能的其他明显未受侵染地区的抹布侵扰变得更加可能。购买Burkard Spore Traps以帮助检测这些地区的黑穗病。初步试验涉及将捕集器放置在侵扰作物和使用光学显微镜进行孢子捕获,但是光学显微镜从时间视角证明耗时和昂贵。开发了分子测定以加速该过程,虽然没有提供定量测定结果,但仍然提供了对Smut的存在的水上态评估。孢子诱捕是在昆士兰州和新南威尔士州许多未受侵染的种植地区的作物中进行的。对斯科西斯皮亚孢子的Smut阳性测定结果被记录在苔藓,Burdekin,沃尔尔格和Maryborough地区。在这些网站中的一些网站的高强度诱捕表明,U. Scitaminea孢子常常是常见的,并且这种疾病很可能在至少一些这些位置侵染作物。 Mareborough地区于2007年11月22日在马里堡地区首次看到粉刷鞭子。

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