Specification and simplified analyzing data from MS/MS spectrometry

摘要

The goal of this work was to design a suitable method for unifying score values from various identification tools of proteins identification in tandem mass spectrometry into a single score value. Output data from the mass spectro-metric method were processed by two independent search tools Mascot and XiTandem. Those were selected especially for their wide applications in proteomic laboratories. Results of both methods were evaluated through a newly designed function and unified to a single valued score that clearly identifies found proteins. The newly designed function of producing of scored values was termed Metascore and implemented into MATLAB computational environment. Given results were successfully tested on selected real data available in public databases of proteomic sequences.