The ability to determine the relative view orientation of objectsfrom their projections has enabled well established tomographictechniques to be employed in the three-dimensional reconstruction ofobjects from images obtained via modalities where the orientation of theobjects cannot be controlled. Most techniques used to determine particleorientation from projections assume that the particle exhibits some formof symmetry. A method is described for the determination of the relativeview orientation of randomly acquired projections of asymmetric objects.It extends upon the projection slice theorem by determining the relativeorientation of projections by the location of lines of intersectionamong the Fourier transforms of the projections in three-dimensionalFourier space. The complete algorithm is described and its efficacy isdemonstrated using real data
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