Solvent Delivery Systems for High Speed Liquid Chromatography

摘要

A major requirement of modern-day liquid chromatography is the capability of its solvent delivery system to precisely deliver the desired flow rate of the mobile phase. This capability must exist over a wide range of flow rates and inlet pressures, and with all useful solvents .Solvent delivery systems may be broadly classified into two basic types, i.e., either constant volume pumps or constant pressure pumps. Both types operate by displacement of liquid from a chamber by a hydraulic piston. The major difference then, is that the piston of a constant volume pump is operated by a mechanical linkage, whereas the piston of a constant pressure pump is operated by gas pressure.These two basic types of solvent delivery systems are discussed with regard to convenience, versatility, performance and durability when subjected to both isocratic and gradient elution applications.