Novel Concepts of 'Niche-Relief' and 'Niche-Voltage' for Stem Cells as a Base of Bone and Hematopoietic Tissues Biomimetic Engineering

摘要

An affect of relief features and quantitative variables of artificial surfaces on structural-functional status of human lung prenatal stromal cells (HLPSC) and remodeling of Balb/c mice bone/bone marrow system have been studied. Implants with rough (Ra>2 μm) calcium phosphate micro-arc coatings have structural-functional sites (micro-regions) named "niches-relief which are necessary for maturation and differentiation of HLPSC into secreting osteoblasts in short-term culture. Maximal remodeling of mouse bone/bone marrow system in 45-day subcutaneous heterotopic test in vivo is also noted under optimal parameter (average index of cellular alkaline phosphatase area to artificial micro-region area is about 43 %) of osteogenic niche in vitro. Probable physical mechanism of osteogenic niche functioning has been determined. It correlates with increasing of electron work function(Φ) supplied with a negative charge of calcium phosphate nanorelief in the sockets (artificial "niches"). "Niche-voltage" concept for bone tissue biomimetic engineering was proposed.