Novel approach to modeling of images emitted by a virtual oligonucleotide library

摘要

The molecular complementarity of two strands of the DNA double helix makes it possible to model changes in DNA sequences basing on their optical properties. The paper is devoted to problems with reliability of fluorescence analysis in investigating DNA chains. A given oligonucleotide library makes a matrix of integrated short sequences which all have their own location and base sequence. The fluorescence effects may be acquired using a CCD camera, converted to digital microimages and stored. In the paper the physical aspects of fluorescence phenomena have been described. The mathematical model for tests tending to determine optimum algorithms of oligonucleotide library detection are proposed. Application of selected algorithms of image processing has been analyzed for conditioning microimages. The results obtained of binarization using digital filtration are presented. The optimal algorithm of image binarization has been shown.