Optimization of Light Transmission through an Excitation-scan Hyperspectral Mirror Array System

摘要

Hyperspectral imaging has numerous applications in a range of fields for target detection. While its originalapplications were in remote sensing, new uses include analyzing food quality, agriculture and medicine,Hyperspectral imaging has shown utility in fluorescence microscopy for detecting signatures from manyfluorescent molecules, but acquisition speeds have been slow due to the need to acquire many spectralbands and the light losses associated with spectral filtering. Therefore, a novel confocal microscope, the 5-Dimensional Rapid Hyperspectral Imaging Platform (RHIP-5D) was designed and is undergoing testing toovercome acquisition speed and sensitivity limitations. The current design utilizes light-emitting diodes(LEDs) and a multifaceted mirror array to combine light sources into a liquid light guide. Initial testsdemonstrated feasibility and we are now working on determining the ideal location of the liquid light guide,LEDs, lenses and mirror array to optimize optical transmission. A computational model was constructedusing Monte Carlo optical ray tracing in TracePro software (Lambda Research Corp.). LED sources weresimulated by importing irradiance properties from the manufacturers’ specifications. Optical properties oflenses were modeled using lens files available from the manufacturer. Analysis of the model includesgeometry and parametric optimization, assessing lens power, mirror angles and location of opticalelements. Initial results show an increase of transmission is possible by up to 20%. Future work willinvolve evaluating the position of the liquid light guide as well as analyzing lens configurations to furtherincrease optical transmission.