Combining Cryo-EM and X-ray Crystallography to Study Membrane Protein Structure and Function

摘要

Membrane proteins perform a wide range of essential functions in all cells of all living organisms, ranging from the sensing, processing and propagation of extrinsic signals, passive or active transport of ions and solutes, creating or utilizing a membrane potential, to the import or secretion of entire proteins. In spite of intense, and increasingly successful efforts in determining membrane protein structures, they still present a formidable challenge in structural biology.