Analysis of Global Expression Profiles of Arabidopsis Genes Under Abscisic Acid and H_2O_2 Applications

摘要

To gain insight into the coordination of gene expression profiles under abscisic acid (ABA) and H_2O_2 applications, global changes in gene expression in response to ABA and H_2O_2 In Aratidopsis seedlings were investigated using GeneChip (Santa Clara, CA, USA) arrays. Among over 24 000 genes present in the arrays, 45! transcripts were found to be significantly Increased, whereas another 221 decreased following H_2O_2 treatment compared with control. Similar to treatment with H_2O_2, ABA treatment elevated the transcription of 391 genes and repressed that of 322 genes. One hundred and forty-three upregulated genes and 75 downregyiated genes were shared between the two treatments and these genes were mainly involved in metabolism, signal transduction, transcription, defense, and resistance. Only two genes, which encode an APETALA2/dehydra-tion-responsive element binding protein (AP2/DREBP) family transcriptional factor and a late embryogenesis-abundant protein, were downregyiated by H_2O_2, but upregulated by ABA. These results suggest that, similar to ABA, H_2O_2 plays a global role in gene transcription of Arahidopsis seedlings. The transcriptional responses induced by the application of exogenous ABA and H_2O_2 overlapped substantially. These two treatments regulated most of the downstream genes in a coordinated manner.