Procedure for determining the presence / absence and Metabolic activity of acidophilic microorganisms measures in function of the Global Gene transcriptional expression of its complement and that comprises a method for obtaining RNA in the necessary quantity and quality analysis, and the C On a microarray multigenomico specific purpose

摘要

Refers to a DNA microarray characterized because it is based on a design that contains 30 to 60 mer oligonucleotide length printed on a support surface or Plate 4x72k format, 8x15k, 4x44k, 8x60k 1x244k or, where such oligonucleotide sequences are selected or F Ragmentos markers and Indicators, standards of SEQ ID no: 1 in 70043,SEQ ID no: 1, SEQ ID no: 1 and 3387, respectively. The microarray is useful in Quantitative Analysis of gene expression of Microorganisms in bioleaching industrial liquid and solid samples. It also refers to a method of Quantitative Analysis of the expression of genes of organisms