OXIDATIVE STRESS IN GSNO REDUCTASE REGULATION IN MICE LUNGS

摘要

In order to explore the molecular regulation of GSNO reductase (GSNOR), effects of environmental formaldehyde exposure and antioxidant -lipoic acid (LA) injection on GSNOR activity and expression in mice lungs were investigated in the present study. Results showed that GSNOR activity was elevated upon 3.0 mg/m3 FA exposure (compared with 0 mg/m3 FA inhaled group, p<0.01) and the elevation was completely inhibited by LA injection (compared with 3.0 mg/m3 FA inhaled group, p<0.01). Meanwhile, GSH levels were significantly decreased in 3.0 mg/m3 FA inhaled group (compared with 0 mg/m3 FA inhaled group, p<0.01), and were regenerated by LA injection (compared with 3.0 mg/m3 FA inhaled group, p<0.01). The expression of GSNOR by using RT-PCR method correlated with the results of GSNOR activity assay. The results indicated that gaseous FA might activate GSNOR, and the activation may be related with the increase of oxidative stress. Since GSNOR has a central role in mediating SNO signaling and asthma development, these findings also suggested that indoor air pollutants such as FA might be key risk factors for the rise in asthma cases.