Identification and Characterization of the XPG Gene of the Frog Rana Grylio Virus

摘要

The RGV_(9808) is one of three Rana Grylio viruses which were isolated from the cultured pig frogs with lethal syndrome obtained from different provinces in China, which belongs to the iridoviridae family. In RGVV_(9808), an open reading frame (ORF), whose deduced amino acid sequence(aa) shows identity with Xeroderma pigmentosum G (XPG) protein, was identified. XPG is one of the nucleotide excision repair (NER) genes, whose encoded protein plays a critical role in NER system as a nuclease. The ORF of RGVV_(9808) has 1092bp in length, G+C content of 60.62% and encodes a putative protein of 363 aa with a predicted molecular mass of 40.6 kDa, whose isoelectric point (pI) is 7.33. Compared with the XPGs found in other 12 species, the higher homology among RGVV_(9808) and the four ranavirus is present as more than 97%, and RGVV_(9808) has the lowest identity with Chilo iridescent virus (CIV), coming to 22.6%. This indicated that there exist large differences among distinct genus viruses, although the gene is identical in iridoviridae family.