Experimental study of laser fluorescence spectrum in determination of ischemic intestinal necrosis

摘要

Abstract: In order to find an objective criteria to determine ischemic intestinal necrosis and present the proof for intestinectomy by surgeons, the fluorescence spectrum on the normal and ischemic intestine, which were stimulated by N$-2$/-laser, was obtained. The intestine ischemic model was established in dogs by ligaturing the blood vessel of mesentery. The fluorescence spectrum was painted by enlarging its signals and inputing them into a computer control system. There is significant difference between normal and pathological tissue: the longer the ischemic duration of the intestine, the more severe its pathological changes and the lower its fluorescence peak. After 6 - 8 hours of ischemic duration going on and complete intestinal necrosis occurred, its fluorescence peak decreased from normal 1.96 - 2.41 to pathological 1. It seems that the laser fluorescence is a simple, quick, accurate, and non-invasive method which can be used in determinations of the ischemic intestinal necrosis. !0