Whole aspect of phosphorus stress metabolisms applying transcrlptomic and proteomic analysis of rice plant

摘要

To evaluate whole aspect of phosphorus (P) stress metabolisms, we analyzed transcriptomic and proteomic alterations in P deficient rice (Oryza sativa L.) plants. Rice plants were cultivated hydroponically with or without P. Total RNA and soluble protein were used for transcriptomic and proteomic analysis, respectively. Generally the responses of gene expression were larger in roots than in the leaves. P deficiency caused a large alteration on gene expression related to not only P metabolism but also C and N metabolism. In roots, many genes related to glycolysis and N acquisition were up- and down-regulated, respectively, which seems to lead enhancement of organic acid exudation by supplying sufficient carbon skeletons. In leaves, many starch metabolism related genes were up-regulated by -P treatment, suggesting a homeostatic contribution to Pi concentration in leaf tissues.