A Computational study of pre-synaptic re-uptake of dopamine on phosphorylation of DARPP-32

摘要

. The complex of adenylate cyclase activates and increases the intracellular levels of cAMP and thereby stimulating cAMP dependent protein kinase and increasing the state of phosphorylation of DARPP-32 (dopamine and cyclic AMP-regulated phosphoprotein, M, = 32,000). DARPP-32 plays an important role in autophosphorylation of calcium calmodulin dependent protein kinaseII (CaMKII) which is crucial for prolongation of long term potentiation (LTP). Here we verified the effect of pre-synaptic re-uptake of dopamine by dopamine transporter on the phosphorylation of DARPP-32 and found that the phosphorylation of DARPP-32 is unaffected by pre-synaptic re-uptake. Further we have seen that the DARPP-32 phosphorylation does not change with the change in the DA or D1R concentrations.