The nanobiosensor on the basis of glucose oxydase enzyme

摘要

The present paper concerns a planar topology for a nanoelectronic transducer based on the glucose oxydase enzyme. The technique of implementation of planar nanostructures for a nanoelectronic biosensor based on the methods of electron-beam lithography is developed. The method of the chemical modification of silicon dioxide surface by epoxysilane was developed and the immobilization of the glucose oxydase enzyme on the surface of planar nanostructure for the nanoelectronic transducer through the linker molecules was realized. The registration procedure of the biochemical signals was developed. A change in the functional state of the immobilized ferment of the glucose oxidase enzyme with the presence of glucose in the test solution was demonstrated.