Isolation and Identification of the Acaricidal Components Extracted from Stellera chamaejasme

摘要

The research of Botanical acaricide is an important aspect of integrated agricultural mite management. Eight kinds of different organic solvents were used for cold-soak extraction from Stellera chamaejasme. Combined with tracking activity, the fraction E7-4 was isolated from ethyl acetate extract using medium-pressure liquid chromatography (MPLC) and preparative HPLC. Using nuclear magnetic resonance, it was determined that fraction E7-4 was Scopoletin. The results showed that the scopoletin in Stellera chamaejasme was the important acaricidal activity ingredient which exhibited contact and systemic activity against Tetranychus cinnabarinus. The Lethal concentrations (LC50) to Tetranychus cinnabarinus were 1.267 mg/mL and 7.388 mg/mL respectively.