New Uses for Immobilized Enzymes and Substrates on Cotton and Cellulose Fibers

摘要

The design, preparation, and application of both immobilized enzymes and enzyme substrates on cotton fibers for biomedical and specialty applications, includes antibacterial fabrics, decontamination wipes, debridement and chronic wound dressing prototypes, and protease detection devices. The molecular design steps of enzyme and enzyme substrate cellulose conjugates is presented. Molecular models of lysozyme- and orgaonphosphorous hydrolase- cellulose conjugates are given as examples of assessing the utility of the biorationally designed fabrics. The chemistry of immobilzing the enzyme or enzyme substrate to the cotton fabric of choice, employs use of crosslinking agents for either aqueous or organic coupling reactions. Assays for assessment bioconjugate activity is pivotal to optimizing the development of the immobilized enzyme or substrate as a product.