Graphene Nanoplatelets-Based Aptamer Biochip for the Detection of Lipocalin-2

摘要

An electrochemical aptasensor platform was developed by using amine-functionalized aptamer that was incorporated onto carbon-printed electrode and modified with graphene nanoplatelets for the detection of lipocalin-2 (LCN-2) protein. The aptamers were covalently bound to the graphene nanoplatelets surface via 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) and N-hydroxysuccinimide (NHS) chemistry and were used as the molecular recognition element for the protein target. Successful interaction between aptamer and LCN-2 protein was ascertained via electrochemical probe, ferro- and ferri-cyanide, Fe(CN)6^{3-/4-, and monitored by means of voltammetric analyses. The developed LCN-2 aptasensor achieved sub-picomolar detection of protein, with the detection limit determined as 0.07 pg ml-1, and a dynamic linear range of 0.1 to 1000 pg ml-1.}