Development of a Cascade Processing Method for Microarray Spot Segmentation

摘要

A new method is proposed for improving microarray spot segmentation for gene quantification. The method introduces a novel combination of three image processing stages, applied locally to each spot image: i/ Fuzzy C-Means unsupervised clustering, for automatic spot background noise estimation, ii/ power spectrum deconvolution filter design, employing background noise information, for spot image restoration, iii/ Gradient-Vector-Flow (GVF-Snake), for spot boundary delineation. Microarray images used in this study comprised a publicly available dataset obtained from the database of the MicroArray Genome Imaging & Clustering Tool website. The proposed method performed better than the GVF-Snake algorithm (Kullback-Liebler metric: 0.0305 bits against 0.0194 bits) and the SPOT commercial software (pairwise mean absolute error between replicates: 0.234 against 0.303). Application of efficient adaptive spot-image restoration on cDNA microarray images improves spot segmentation and subsequent gene quantification.