Inhibition of G-Quadruplex Helicase Activity As Determined by Surface Plasmon Resonance

摘要

The ability of G-quadruplex interactive small molecules to inhibit SV40 Large T-Antigen G-quadruplex helicase activity was determined using a surface plasmon resonance based assay. Unwinding of a physiologically relevant intramolecular G-quadruplex DNA substrate was completely inhibited by one novel porphyrin derivative under these conditions. This SPR-based method represents an important strategy for comparing helicase inhibition effectiveness.