INTEGRATION AND APPLICATION OF A SURFACE PLASMON SENSOR ARRAY ON-CHIP

摘要

A microfluidic device with an embedded surface plasmon resonance (SPR) sensor array was developed. The sensing elements were nanohole arrays, differentiated by periodicity. The device was applied to detection of concentration across a gradient, and the detection of surface binding events. To determine sensitivity and for the gradient-based detection experiments, glucose solutions of known refractive index were employed. Consecutive surface binding events were monitored in the flow-through assembly of a monolayer (cysteamine) with a linker (biotin) and a protein (streptavidin).