Biofabrication of osteochondral grafts via 3D printing of cell-laden microcarriers in a gelatin methacrylamide/gellan gum bioink

摘要

MCs-laden GelMA/gellan gum was proven to be a promising composite material for biofabrication. MCs acted as a mechanical reinforcement for the soft gel. Encapsulation of MC-MSCs complexes -with improved cell adhesion and cell-cell contacts- was effective to improve osteogenic differentiation and to support bone matrix deposition. MCs culture is of great interest for MSCs expansion and scaling-up of bone-like tissues bioprinting. These are key findings to build advanced constructs for bone and zonal osteochondral regeneration.