Effect of Salt (NaHCO_3) Stress on Biochemical Parameters of Moms Alba (Sujanpuri) In Vitro

摘要

Salt accumulation is the primary factor depressing the yield of cultivated crops. Mulberry (Morus alba L) is practically the sole food for the silkworm and its foliage forms the basis of commercial sericulture. A major determining factor of productivity and profitability in sericulture is the yield of qualitative mulberry foliage, which greatly varies under different agro climatic conditions. Salt problems are generally more pronounced in mulberry gardens of tropical and subtropical regions. A highly biotic approach to overcome these limitations is selection of salt tolerant lines among the existing varieties. Growing of salt tolerant saplings of mulberry would not only help sericulture industry but also potentially could lead to the utilization of salt affected lands.rnIn vitro experiments were conducted to study the effect of alkalinity stress by applying sodium bicarbonate salt (NaHCO ) to the saplings of Moms alba (var. Sujanpuri) cultured from nodal explants with axillary buds. For shoot multiplication 2.5 mg/l of 6 benzylaminopurine (BAP) with 0.3mg/l gibberellic acid (GA_3) was used and rooting was induced with 1.0 mg/l of Indolebutyric Acid (IBA). For induction of alkalinity stress, NaHCO_3 salt was added in the culture medium in three concentrations i.e. 3.57, 20.0 and 59.0 mM with pH 6.2, 7.2 and 8.2 and EC 5.11,6.70 and 9.55 m mohs/cm respectively. The cultured biomass analyzed for proline, protein, sugars and chlorophyll content resulted in increased proline, protein and sugars at concentrations 3.57 mM (pH= 6.2) and 20 mM (pH= 7.2) and decreased the level of chlorophyll at all stress regimes. However at higher concentrations i.e., 59 mM (pH= 8.2) the protein and sugar also got decreased.