Two-dimensional fluorescence spectroscopy for online monitoring of chromatographic separation of molasses

摘要

Abstract: Monitoring and control of industrial or biotechnological processes invariably require reliable and fast analytical systems. Two-dimensional spectral fluorescence allows real time automatic measurements directly inside the process and provides a continuous stream of information compared to discrete information available from repeated sampling and offline analysis. Normally, chromatographic molasses desugarization processes are typically monitored with a combination of an online-refractometer, polarimeter and the measurement of density and conductivity. Additional information of the separation profile could be obtained by multiple sampling during the separation cycle followed by offline laboratory analysis. An optical sensor (BioView$+R$/) allows online fluorescence measurements for a continuous monitoring directly at the outlet of the separation columns. It was the aim to predict the amino acid serine during the chromatographic cycle. Based on fluorescence, it was possible to monitor a fluorophor, which eluted a few minutes before the serine fraction during molasses desugarization. The application of fluorescence measurements for monitoring and control of chromatographic separation process may improve yield and purity of the separating fractions and can lower the costs for the next downstream processing of by-products. !9