Monitoring of interaction of hemoglobin and glucose molecules by spectral method

摘要

This study is focused on the determination of the absorbance of oxyhemoglobin solutions at different concentrations of glucose (from 0 to 1000 mg/dl with a step 100 mg/dl) and hemoglobin (3.2g/l) incubation with glucose from 3 hours to a few weeks. The absorbance was determined within the wavelength range from 500 to 900 nm. Measurements of the absorbance spectra have been performed using a double-beam double-wavelength spectrophotometer.