Lag time changes between capillary blood glucose and in vivo skin interstitial glucose levels by HATR-FTIR spectroscopy

摘要

Method of the lag/latency time (LT) measurement, calculation and interpretation can be simultaneously applied to study in vivo glucose diffusion from the capillary to the skin tissue, to calibrate spectroscopically measured glucose levels during real-time glucose monitoring of dynamic processes in the skin tissue and to study glucose optical properties in the living skin tissue. Based on previous reports on determining interstitial glucose levels and their LT's by HATR-FTIR spectroscopy, here the LT was calculated for each glucose absorbance level at about 1030-41,1080,1118 and 1153 cm~(-1) during oral glucose tolerance test (OGTT) with different doses (5g, 20g, 75g). The LT showed dose-dependency and described intra-/inter-subject changes of skin glucose dynamics in healthy and diabetes subjects. The time required for glucose to diffuse from the capillary to the skin tissue was shorter in a diabetes subject, than in a healthy subject, independently on intaken dose of glucose. Nevertheless, in both subjects the LT changes ranged within 0-50 minutes. Measurement of the LT demonstrated a potential to provide insight to healthy and diabetic glucose dynamics between the blood and interstitial fluid compartments in the upper layer of the skin tissue. Also, the LT might be regarded as a method to calibrate dynamic measurements of glucose in vivo by this spectroscopy method and to characterize living skin tissue glucose optical properties.