OPTIMIZATION OF MATRIX SOLID PHASE DISPERSION EXTRACTION PROCEDURE FOR THE ANALYSIS OF POLYBROMINATED DIPHENYL ETHERS IN HUMAN PLACENTA

摘要

Characterization of pre natal exposure to hazardous chemicals most often rely upon the analysis of cord blood. However, human placenta is an appropriate alternative with noteworthy advantages. Owing to the analytical challenges, reports on placental levels of toxic chemicals are limited. The purpose of this study was to establish a reliable, cost effective and a relatively fast and easy method to extract polybrominated diphenyl ethers (PBDEs) from human placenta. The matrix solid phase dispersion (MSPD) method was optimized. Different sorbents, sample conditions, grinding methods, elution solvents, and single and repeated extractions were compared for their effects on the extraction efficiency. With the optimized method, the recovery of PBDEs was 91- 114% for the spiked placenta samples and 89-115% for a standard reference material. The extraction efficiency of the MSPD method was found to be comparable with the Soxhlet method and superior to that using a liquid extraction method.