Reconstruction of fluorophore distribution for fluorescence diffuse tomography based on hybrid model

摘要

We propose a novel method for reconstruction of fluorophore spatial distribution in a highly scattering object imaged by means of fluorescence diffuse tomography. The method is based on algebraic reconstruction principle combined with a new theoretical model of light propagation in biotissues and Monte Carlo simulations. Monte Carlo simulations are also implemented for initial data calculations in approbation of the method. The reconstruction method was tested both for simulated and experimental initial data. We demonstrate that the method provides determination of center position of fluorescent inclusion in transversal direction with the accuracy of 1 mm, in-depth direction with the accuracy of 1.5 mm and determination of size of fluorescent inclusion with the accuracy of 1.5 mm.