Scanning Fiber Endoscope with multiple fluorescence-reflectance imaging channels for guiding biopsy

摘要

We report on the performance and detection sensitivity of a newly developed wide-field, multi-spectral fluorescence-reflectance scanning fiber endoscope (SFE), which was designed for imaging of fluorescence labeled molecular probes during endoscopy for early cancer detection. The device contains three fluorescence excitation sources: 448 nm (blue), 488 nm (blue/green) and 642 nm (red). Three fluorescence detection channels for quantitative diagnostic imaging and one reflectance channel for intraoperative navigation are available. Using test targets, concurrent (30Hz), wide-field (80°) and high resolution (50μm) imaging was confirmed. The detection sensitivity was evaluated at nanomolar level (5nmol/L) dye concentrations. Meanwhile, simultaneous detection of blue, green, and red/NIR fluorescence with a grayscale reflectance background was demonstrated for concurrent multispectral fluorescence endoscopic imaging.