The three-dimensional imaging of microspheres in confocal and conventional polarization microscopes

摘要

Images of the microspheres are studied in three-dimensions by using the confocal and conventional scantling polarization microscopes. It is found that the polarization of the detected signals is mainly parallel to the initial polarization which is due to the high extinction coefficient of the confocal system. Arc pairs are observed at the edge of the microspheres with the conventional polarization microscope with a crossed analyzer. Theoretical analysis are given by using the vector field theory and the image formations of the two systems.