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CONFOCAL MICROSCOPE AND METHOD FOR FORMING THREE-DIMENSIONAL IMAGE BY USING THE SAME CONFOCAL MICROSCOPE

机译:共焦显微镜和通过使用相同的共焦显微镜形成三维图像的方法

摘要

PROBLEM TO BE SOLVED: To form and rotate a three-dimensional distortion-free image without using an expensive computer capable of high-speed calculation. ;SOLUTION: This microscope is equipped with a light source 2, a two-dimensional scan unit 10 which makes a two-dimensional scan on a sample 4 with the light from the light source 2, an objective 20 arranged between the scan unit 10 and sample 4, an objective driving part 21 which moves the objective 20 and sample 4 relatively along the optical axis of the objective 20, a pinhole 31 which is provided at a position conjugate to the focal plane of the objective 20, and a fluorescent light detector 40 for detecting light from the sample 4 which has been passed through the pinhole 31. In this case, the scan by the two-dimensional scan unit 10 and the movement by the objective lens driving part 21 along the optical axis are performed at the same time and a control part 50 is provided which controls the moving speed and moving direction of the objective lens driving part 21 as to the scanning speed of the two-dimensional scan unit 10, thereby obtaining a segmental image of the sample 4 perpendicular to an axis slanting to the optical axis.;COPYRIGHT: (C)1998,JPO
机译:解决的问题:无需使用能够进行高速计算的昂贵计算机,即可形成并旋转三维无畸变图像。 ;解决方案:该显微镜配备有光源2,二维扫描单元10,该二维扫描单元10用来自光源2的光对样品4进行二维扫描,物镜20布置在扫描单元10和扫描单元10之间。样品4,使物镜20和样品4沿物镜20的光轴相对移动的物镜驱动部21,设在与物镜20的焦平面共轭的位置的针孔31,以及荧光检测器图40中示出了用于检测已经穿过针孔31的来自样本4的光的情况。在这种情况下,二维扫描单元10的扫描和物镜驱动部21沿着光轴的移动是在同一时间进行的。时间,并且提供控制部件50,该部件控制物镜驱动部件21的移动速度和移动方向相对于二维扫描单元10的扫描速度,从而获得样本4的分割图像。倾斜于光轴的垂直轴;版权:(C)1998,JPO

著录项

  • 公开/公告号JPH10161034A

    专利类型

  • 公开/公告日1998-06-19

    原文格式PDF

  • 申请/专利权人 NIKON CORP;

    申请/专利号JP19960336421

  • 发明设计人 HAKOZAKI HIROYUKI;

    申请日1996-12-02

  • 分类号G02B21/00;G01B9/04;

  • 国家 JP

  • 入库时间 2022-08-22 03:07:16

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