Determining the reduced scattering of skin in vivo using sized - fiber reflectometry

摘要

Sized-fiber reflectometry describes a device and method for measuring absorption and reduced scattering of tissue using optical fibers with different diameters. The device used in this paper consists of two fibers with diameters of 200 and 600 micron. Each fiber emits and collects its own backscattered light. Monte Carlo simulations tabulating the diffuse reflectance collected by 200 and 600 micron fibers in a semi-infinite homogenous media are presented for an absorption,μ_a range of 0.2-30 cm~(―1) and a reduced scattering,μ′_s range of 10-200 cm~(―1). The diffuse reflectance collected by a 600 micron fiber may be approximated by a linear relation to the 200 micron fiber. An empirical relation is derived relating the reduced scattering coefficient to the diffuse reflectance collected with 200 and 600 micron fibers. The sensitivity of the relation is determined for changes in each fiber measurement. Finally, in vivo diffuse reflection measurements and reduced scattering coefficient of skin are presented using the aforementioned fiber sizes with a wavelength range of 400-800 nm.