PHOTODYNAMIC LASER THERAPY IN THE TRANSPARENCY REGION OF BIOTISSUES USING TRICARBOCYANINE DYES AS PHOTOSENSITIZERS

摘要

Investigation of the spectral properties of polymethine dyes in organic solvents, water, nutritions media and cell cultures at various concentrations has demonstrated that the tumor cells contain monomers of the dye molecules. Pharmacokinetics of the different dyes has been studied both in vivo and in vitro. After incubation of a cell culture in the presence of a nontoxic dose of the dyes their concentration in rumor cells is rather high, varying for different preparations from 10~(-11) o 2.1·10~(-9) mol per million cells. The biggest phototoxicity in vitro has been observed for polymethine dyes with indolenine end groups. In vivo concentration of the dyes in tumor tissues was higher then in normal muscle tissues ranging from 1.2 to 3.6 for different kinds of tumor. Photobleaching of dyes during photodynamic treatment was observed. The photodynamic treatment with some polymethine dyes caused tumor tissue necrosis up to 20 mm in depth.