NANOLITER AND PICOLITER LIQUID HANDLING FOR LIFE SCIENCE APPLICATIONS

摘要

Acoustic droplet ejection (ADE) can gently and precisely aliquot nanoliter and picoliter liquid volumes without any physical contact with the solution being transferred. A wide variety of solvents, DNA, proteins and live mammalian cells can be dispensed by ADE without detectable loss of activity or viability. Picoliter~R ADE technology couples a transducer to a fluid container, including SBS-standard microplates, to deliver focused acoustic energy into the fluid and induce droplet ejection. For containers holding two immiscible fluids, acoustic energy focused at the fluid-fluid interface generates droplets composed of both fluids. Applications of ADE to solutions, cell suspensions and multi-fluid droplets for small-scale assays and particle formation will be discussed.