Femtosecond laser scalpel technology for proteomic mass spectrometry

摘要

Tandem mass spectrometry is a widely used tool for molecular structure determination of biomolecules. Advances in proteome analysis depend on the development of improved methods for ion activation that yield greater sequence information, with selective control over the fragmentation chemistry. A novel activation method, femtosecond laser-induced ionization/dissociation, is described here, and compared to collision induced dissociation through the analysis of three peptides.