Photoacoustic imaging of tumor angiogenesis

摘要

Photoacoustic imaging is a hybrid imaging modality that is based on the detection of acoustic waves generated by absorption of pulsed light by tissue chromophores such as hemoglobin in blood. Serial photoacoustic imaging has been performed over a 10-day period after subcutaneous inoculation of pancreatic tumor cells in a rat. The images were obtained from ultrasound generated by absorption in hemoglobin of short laser pulses at a wavelength of 1064 ran. The ultrasound signals were measured in reflection mode using a double-ring photoacoustic detector. A correction algorithm has been developed to correct for scanning and movement artifacts during the measurements. Three-dimensional data visualize the development and quantify the extent of individual blood vessels around the growing tumor, blood concentration changes inside the tumor and growth in depth of the neovascularized region.