Effect of HeNe laser irradiation on the phagocytosis of macrophages in the immune organs in mice

摘要

In order to study the effect of HeNe laser on macrophages phagocytosis, the area over liver and spleen of the mice was irradiated with HeNe laser at the dosage of 63.7J/cm~2 for 3,5,6 and 10 days respectively, 5 min each day, then observing the phagocytosis of macrophages of the immune organs after tyrpan blue injecting live mice, and made quantitative analysis of macrophages phagocytosis by image analysis system. The results showed that the four indexes: number of trypan blue granule (GN), area of granule (GA), ratio of granule area to cell area (Ga/CA) and granule integral optical density (IOD) at irradiated groups were higher than that of control group (P<0.01). The study indicated that HeNe laser with approapriate dosage can activate macrophages of the immune organs, and enhance organic immunity.