Effects of Culture Condition on Solubilization of Coal by Microorganisms

摘要

Biosolubilization of an Australian lignite was investigated by using Streptomyces viridosporus and Poria cocos. In order to solubilize coals effectively they were pretreated by nitric acid both in surface and liquid cultures. The effects of various carbon, nitrogen and metal sources on overall solubilization were also studied. Solubility increased with the addition of urea for S.viridosporus, and peptone and tryptone for P.cocos. However carbon and metal sources had little or negative effects on solubilization. Maximum amount of coal solubilized was 85 %(w/w) in a batch fermentation culture. Characteristics of the solubilized coal were compared with those of original coal and pretreated coal by the approximate and ultimate composition analysis, and IR-spectrum analysis. The spectroscopic results showed that the mechanism of coal solubilization was caused by continuous oxidation.