SAM Method as an Approach to Select Candidates for Human Prostate Cancer Markers

摘要

In order to select gene markers among differentially expressed transcripts identified from tumoral prostate, we have applied a filter and Significance Analysis of Microarrays (SAM) as the feature selection method on a previously normalized dataset of DNA microarray experiments reported by Reis et al., 2004 (Oncogene 23:6684-6692). Twenty seven samples with different degrees of tumor differentiation (Gleason scores) were analyzed. SAM was run using either two-class, unpaired data analysis with Gleason 5-6 and Gleason 9-10 samples, or multiclass response analysis with an additional category of Gleason 7-8. Both strategies revealed a promising set of transcripts associated with the degree of differentiation of prostate tumors.