Timed release of cerebrolysin using titanate nanospheres induces neuroprotection in Parkinson's Disease

摘要

In this innovation, we report the potential neuroproetctive effects of slow release of Cerebrolysin, a multimodal drug comprising a balance composition of several neurotrophic factors and active pepride fragments on brain pathology induced by Parkinson's Disease (PD). PD like symptoms was induced in the mice by intraperitoneal injections of or 1-metyl-4-fenyl-1,2,3,6-tetrahydropyridin (MPTP, 20 mg/kg) daily within 2-h intervals for 5 days. A significant decrease in dopamine (DA) and its metabolites 3,4-Dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) along with the number of tyrosine hydroxylase (TH) positive cells in the Substantia Nigra Pars Compacta (SNpc) and striatum (STr) confirmed the validity of our model Our observations showed that timed release of CBL using titanate nanospheres (TiNS) (in a dose of 3 ml/kg, i.v.) when given after 2-days of MPTP administration for 5 days resulted in a marked increase in TH-positive cells in the SNpc and STr as compared to normal CBL. Also TiNS-CBL resulted in significantly higher levels of DA, DOPAC and HVA in SNpc and STr on the 8th day as compared to normal CBL. Tehse observations are the first to point out that timed release of TiNS-CBL has far more superior neuroprotective effects in PD than normal CBL, not reported earlier.