Investigation of androgen effects on prostate cancer cell lines by near-infrared Raman microspectroscopy

摘要

Abstract: Near IR Raman spectroscopy was used to investigate the effect of synthetic androgen R1881 on the androgen- responsive cell line LNCaP. The comparison between Raman spectra of the cell sunder androgen deprived conditions and in the presence of different concentrations of R1881 shows changes mainly in the concentration of lipids and DNA content. The androgen-unresponsive prostate cell line PC3 was used as a control. Our results demonstrate that in LNCaP cells R1881 induces an intracellular accumulation of lipids and leads to a relative decrease in DNA content. These changes could potentially be used as criteria to differentiate between responsive and unresponsive cell lines because they were not observed in the androgen unresponsive cell line PC3. We have also measured Raman spectra of lipid droplets directly in single living LNCaP cells, grown in the absence or in the presence of R1881 by Raman spectrometry. These droplets accumulate in cells grown in the presence of R1881. Our results show indeed that the main components in droplets were lipids and suggest that the surrounding cytoplasm does not significantly contribute to these Raman spectra. The major classes of lipids droplets affected by androgen were triglyceride and cholesterol linoleate. !13