Quantitative analysis of amyloid mass by segmentation of microPET and CT images

摘要

Immunoglobulin light chain amyloidosis (AL) is characterized by the deposition of light chain components as insoluble fibrils in tissues and organs leading to dysfunction and death and is the most common form of peripheral amyloid disease in humans. Presently, there are no means available in the USA to image specifically these deposits and thus ascertain the presence or extent of disease. To this end we have developed an experimental murine model of localized AL and used this to evaluate a radioiodinated amyloid fibril-reactive antibody, 11-1F4. The goal of the present study was to validate the experimental model by correlating the accumulation of ¹²⁴I-11-1F4 antibody in the amyloid, using segmented PET and CT images, with lesion mass, volume, and tissue activity.